Active caspase-3 detection to evaluate apoptosis induced by Verbena officinalis essential oil and citral in chronic lymphocytic leukaemia cells
نویسندگان
چکیده
منابع مشابه
The assessment of caspase activation during apoptosis of malignant cells in B-cell chronic lymphocytic leukaemia
In this study we analysed caspase activation in peripheral blood and bone marrow cells of B-cell chronic lymphocytic leukaemia (B-CLL) exposed to apoptosis both spontaneous and induced via external and internal pathway. We used the method based on detection of activated caspases with FAM-VADFMK that is the fluorochrome labelled inhibitor of caspases and flow cytometry technique. We detected the...
متن کاملChemical Composition, Antibacterial and Antioxidant Activities of the Essential Oil Isolated from Verbena officinalis
The essential oil of Verbena officinalis growing in Iran was extracted using hydro-distillation method and the antibacterial and antioxidant activities were evaluated. The antibacterial activity of the essential oil against a Gram-positive (S. aureus ATCC25923) and Gram negative (E. coli ATCC 25922) were examined. The results obtained revealed that the essential oil exhibited variable levels of...
متن کاملSelective IAP inhibition results in sensitization of unstimulated but not CD40-stimulated chronic lymphocytic leukaemia cells to TRAIL-induced apoptosis
Despite recent advances in therapy, chronic lymphocytic leukaemia (CLL) remains incurable and new treatment strategies are therefore urgently required. Inhibitor of apoptosis proteins (IAPs) are over-expressed in CLL, suggesting both a role in disease pathogenesis and the potential for therapeutic targeting. To explore these questions, we evaluated the effects on primary CLL cells of AZD5582, a...
متن کاملChronic lymphocytic leukaemia.
Chronic lymphocytic leukaemia is the commonest of the leukaemias and occurs most often in the elderly. The identification of presenting features that correlate adversely or favourably with survival has allowed the rational development of new treatment strategies.
متن کاملChronic lymphocytic leukaemia (CLL)
Typically, proliferation of mature small lymphocytes of normal morphology; lymphocytes with more abundant cytoplasm may be present. When prolymphocytes are 10% or greater they are classified as 'chronic lymphocytic leukaemia-prolymphocytic leukaemia'. The main immunophenotypic features that define BCLL are: the predominant population shares B-cell markers CD19, CD20, and CD23 with the CD5 antig...
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ژورنال
عنوان ژورنال: Revista Brasileira de Farmacognosia
سال: 2011
ISSN: 0102-695X
DOI: 10.1590/s0102-695x2011005000082